A simple and rapid external standard calibration HPLC methodfor determination of lumefantrine in dried blood spot samplesfrom malaria patients in Botswana

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dc.contributor.authorMassele, Amos Y.
dc.date.accessioned2024-04-05T12:18:58Z
dc.date.available2024-04-05T12:18:58Z
dc.date.issued2024-01
dc.description.abstractAbstract A simple external calibration liquid chromatography-diode array detector method was developed, validated, and applied for the determination of lumefantrine (Lum) in dried blood spot (DBS) samples collected from malaria patients in Botswana. The samples were validated in accordance with the United States Food and Drug Administration guidelines for bioanalytical methods after sample preparation using solid-liquid extraction. Separation was achieved using an XTerra C18 column (50 × 4.6 mm, 5 μm), and a binary solvent system of acetonitrile and water adjusted to pH 2.3 was used as the mobile phase. The validated method was applied for the determination of Lum in DBS samples collected from malaria patients infected with Plasmodium falciparum in Botswana. The calibration curve was linear between 0.5 and 12 μg/mL with a coefficient of determination (R2 ) of 0.9996. The limit of detection and the lower limit of quantification were 0.5 and 1.4 μg/mL, respectively. The efficiency of extraction measured as percentage recovery ranged between 84.2% and 107.8% at the three quality control (QC) levels, that is, low QC, mid QC, and high QC. In conclusion, data suggest that the method is suitable for the determination of trace Lum in biofluids and can also be used for therapeutic drug monitoring and pharmacokinetic profiling. Keywords: HPLC; LLOQ; dried blood spot; lumefantrine; malaria.en_US
dc.identifier.citationBrooks, B.O., Paganotti, G.M., Massele, A., Sepako, E., Adiukwu, P. and Sichilongo, K.F., 2024. A simple and rapid external standard calibration HPLC method for determination of lumefantrine in dried blood spot samples from malaria patients in Botswana. Biomedical Chromatography, 38(1), p.e5762.en_US
dc.identifier.otherDOI: 10.1002/bmc.5762
dc.identifier.urihttp://hdl.handle.net/123456789/1334
dc.language.isoenen_US
dc.publisherBiomedical Chromatographyen_US
dc.subjectHPLCen_US
dc.subjectLLOQen_US
dc.subjectDried blood spoten_US
dc.titleA simple and rapid external standard calibration HPLC methodfor determination of lumefantrine in dried blood spot samplesfrom malaria patients in Botswanaen_US
dc.typeArticleen_US

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