Quantification of Plasmodium falciparum Histidine-Rich Protein-2 in Cerebrospinal Spinal Fluid from Cerebral Malaria Patients
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Date
2014
Journal Title
Journal ISSN
Volume Title
Publisher
The American journal of tropical medicine and hygiene
Abstract
Abstract
A cerebrospinal fluid (CSF) biomarker for cerebral malaria (CM) has not been validated. We
examined the detection, semi quantification, and clinical use of the Plasmodium falciparum
histidine-rich protein-2 (PfHRP-2) as a parasite antigen biomarker for CM. The PfHRP-2 was
detected in archival CSF samples from CM patients from Tanzania both by a newly developed
sensitive and specific immuno-polymerase chain reaction (72 of 73) and by rapid diagnostic tests
(62 of 73). The geometric mean PfHRP-2 CSF concentration was 8.76 ng/mL with no differences
in those who survived (9.2 ng/mL), those who died (11.1 ng/mL), and those with neurologic
sequelae (10.8 ng/mL). All aparasitemic endemic and nonendemic control samples had
undetectable CSF PfHRP-2. In a separate group of 11 matched plasma and CSF cerebral malaria
patient samples, the ratio of plasma to CSF PfHRP-2 was 175. The CSF PfHRP-2 reflects elevated
plasma PfHRP-2 rather than elevated CM-specific CSF ratios, falling short of a validated
biomarker.
Description
Keywords
Plasmodium, Falciparum, Protein-2
Citation
Mikita, K., Thakur, K., Anstey, N.M., Piera, K.A., Pardo, C.A., Weinberg, J.B., Mukemba, J., Florence, S., Mwaikambo, E.D., Granger, D.L. and Sullivan, D.J., 2014. Quantification of Plasmodium falciparum histidine-rich protein-2 in cerebrospinal spinal fluid from cerebral malaria patients. The American journal of tropical medicine and hygiene, 91(3), pp.486-492.