Quantification of Plasmodium falciparum Histidine-Rich Protein-2 in Cerebrospinal Spinal Fluid from Cerebral Malaria Patients

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dc.contributor.authorMwaikambo, Esther D.
dc.contributor.authorKalabamu, Florence S.
dc.date.accessioned2020-10-23T06:04:56Z
dc.date.available2020-10-23T06:04:56Z
dc.date.issued2014
dc.description.abstractAbstract A cerebrospinal fluid (CSF) biomarker for cerebral malaria (CM) has not been validated. We examined the detection, semi quantification, and clinical use of the Plasmodium falciparum histidine-rich protein-2 (PfHRP-2) as a parasite antigen biomarker for CM. The PfHRP-2 was detected in archival CSF samples from CM patients from Tanzania both by a newly developed sensitive and specific immuno-polymerase chain reaction (72 of 73) and by rapid diagnostic tests (62 of 73). The geometric mean PfHRP-2 CSF concentration was 8.76 ng/mL with no differences in those who survived (9.2 ng/mL), those who died (11.1 ng/mL), and those with neurologic sequelae (10.8 ng/mL). All aparasitemic endemic and nonendemic control samples had undetectable CSF PfHRP-2. In a separate group of 11 matched plasma and CSF cerebral malaria patient samples, the ratio of plasma to CSF PfHRP-2 was 175. The CSF PfHRP-2 reflects elevated plasma PfHRP-2 rather than elevated CM-specific CSF ratios, falling short of a validated biomarker.en_US
dc.identifier.citationMikita, K., Thakur, K., Anstey, N.M., Piera, K.A., Pardo, C.A., Weinberg, J.B., Mukemba, J., Florence, S., Mwaikambo, E.D., Granger, D.L. and Sullivan, D.J., 2014. Quantification of Plasmodium falciparum histidine-rich protein-2 in cerebrospinal spinal fluid from cerebral malaria patients. The American journal of tropical medicine and hygiene, 91(3), pp.486-492.en_US
dc.identifier.otherdoi:10.4269/ajtmh.14-0210
dc.identifier.urihttp://hdl.handle.net/123456789/702
dc.language.isoenen_US
dc.publisherThe American journal of tropical medicine and hygieneen_US
dc.subjectPlasmodiumen_US
dc.subjectFalciparumen_US
dc.subjectProtein-2en_US
dc.titleQuantification of Plasmodium falciparum Histidine-Rich Protein-2 in Cerebrospinal Spinal Fluid from Cerebral Malaria Patientsen_US
dc.typeArticleen_US

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