Polymerase chain reaction–single strand conformation polymorphism analyses of nuclear and chloroplast DNA provide evidence for recombination, multiple introductions and nascent speciation in the Caulerpa taxifolia complex

Mgone, Charles S.

Polymerase chain reaction–single strand conformation polymorphism analyses of nuclear and chloroplast DNA provide evidence for recombination, multiple introductions and nascent speciation in the Caulerpa taxifolia complex

dc.contributor.author	Mgone, Charles S.
dc.date.accessioned	2020-03-17T10:46:57Z
dc.date.available	2020-03-17T10:46:57Z
dc.date.issued	2002
dc.description.abstract	Independent lines of evidence support an Australian origin for the Mediterranean populations of the tropical alga Caulerpa taxifolia. To complement previous biogeographical studies based on nuclear rDNA internal transcribed spacer (ITS), a new chloroplast marker was developed Ñ the cp 16S rDNA intron-2. Sequence variability for both nuclear and chloroplast markers were assessed in 110 individuals using single strand conformation polymorphism. Comparison of intrapopulation genetic diversity between invasive Mediterranean and ÔnativeÕ Australian populations revealed the occurrence of two divergent and widespread clades. The first clade grouped nontropical invasive populations with inshore-mainland populations from Australia, while the second clustered all offshore-island populations studied so far. Despite our finding of nine distinct nuclear and five distinct chloroplast profiles, a single nucleocytoplasmic combination was characteristic of the invasive populations and sexual reproduction was found to be very rare. C. taxifolia is clearly a complex of genetically and ecologically differentiated sibling species or subspecies.	en_US
dc.identifier.citation	Meusnier, I., Valero, M., Destombe, C., Gode, C., Desmarais, E., Bonhomme, F., Stam, W.T. and Olsen, J.L., 2002. Polymerase chain reaction–single strand conformation polymorphism analyses of nuclear and chloroplast DNA provide evidence for recombination, multiple introductions and nascent speciation in the Caulerpa taxifolia complex. Molecular Ecology, 11(11), pp.2317-2325.	en_US
dc.identifier.other	https://doi.org/10.1046/j.1365-294X.2002.01627.x
dc.identifier.uri	http://hdl.handle.net/123456789/357
dc.language.iso	en	en_US
dc.publisher	Molecular ecology	en_US
dc.subject	Asexual reproduction	en_US
dc.subject	Chloroplast 16S intron	en_US
dc.subject	Invasive species	en_US
dc.title	Polymerase chain reaction–single strand conformation polymorphism analyses of nuclear and chloroplast DNA provide evidence for recombination, multiple introductions and nascent speciation in the Caulerpa taxifolia complex	en_US
dc.type	Article	en_US