Urinary tract infection: prevalence pathogens and antimicrobial susceptibility pattern among febrile children at Mwananyamala Hospital Tanzania

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Date

2013

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Hubert Kairuki Memorial University

Abstract

Background: Urinary tract infection (UTI) is a common and important cause of morbidity in the pediatric population in developing countries. Prevalence rates of urinary tract infection in children range from 3.3% in the United States to 39.7% in Northwestern Tanzania. Diagnosis of Urinary Tractjnfection in children is difficult to establish in our setup, therefore in resource-limited settings most children with Urinary Tract Infection are either misdiagnosed or given empiric treatment without laboratory confirmation of the infection. Moreover, many uropathogens are developing resistance to antibiotics recommended by World health Organization (WHO) to treat urinary tract infection. The magnitude, etiology and antimicrobial susceptibility of Urinary Tract Infection in Tanzanian febrile children are not well defined. All published studies to date on the prevalence and etiology of UTI in febrile children have not included a control group which is necessary to investigate false positives due to poor collection procedures or a latent period before the sample is inoculated to the agar growth media. The urine collected for urinalysis or microscopic needs to be tested as soon as possible so as to prevent multiplications of organisms. If the urine has to be cultured, it should be refrigerated during transit and held refrigerated until cultured. Objective: The objectives of this study was to determine the prevalence of Urinary Tract Infection, aetiologic pathogens and antimicrobial susceptibility of these pathogens in febrile children aged two to five (2-5) years in Mwananyamala District Hospital. Methodology: This was a Cross-sectional descriptive hospital-based study which was conducted among febrile children aged two to five years. Febrile and afebrile children were consecutively recruited from the paediatric and the Reproductive and vii Child health (RCH) clinics at Mwananyamala District Hospital. The study was carried out for six months, i.e. from July to December 2012. Urine was collected by use of clean catch method into a sterile container. The diagnosis of Urinary Tract Infection was confumed by bacteriological culture of urine specimen. If bacterial uropathogens was isolated from the urine specimen, antimicrobial susceptibility testing was performed using discs for the following antimicrobials: Cotrimoxazole, Amoxicillin, Erythromycin, Gentamycin, Ceftriaxone, Ciprofloxacin and Amikacin Results: A total of 556 children fulfilled the eligibility criteria and were enrolled into the study. Of these 370 (66.5%) were febrile and 186 (33.5%) were afebrile. Among the 370 febrile children 29 children were confirmed to have urinary tract infection by urine culture, giving an overall prevalence rate of 7.8%. Females were noted to have higher prevalence of UTI than males, however, the difference was not statistically significant (P^ 0.418). Of the 186 afebrile children 11 were confirmed by urine culture to have urinary tract infection giving an overall prevalence rate of 5.9%. Febrile children were noted to have higher prevalence of urinary tract infection than afebrile children, however the difference was not statistically significant (P= 0.729). Escherichia coli was the most commonly isolated organism accounting for 38%, followed by Klebsiella species which accounted for 21%. Other organisms isolated included Proteus species, Pseudomonas species and Staphylococcus aureus. Resistance rates of the isolated uropathogens to, Co-trimoxazole, Erythromycin and Amoxillin were 100%, 89.7% and 86.2%, respectively. Gentamycin and Ceftriaxone had slightly lower resistance rates of 48% and 57% respectively while Amikacin and Ciprofloxacin had least resistance rates of 0% and 6.9%respectively. Conclusion: Prevalence of Urinary Tract Infection among febrile children aged two to five (2-5) years attending the Paediatric and the Reproductive and Child Health Clinics in Mwananyamala District Hospital, Dar es Salaam is 7.8%. The commonest bacterial Uropathogens isolated fi^om the urine of these children were Exoli and Klebsiella Species. The isolated bacterial uropathogens showed a relatively high resistance to Amoxillin, Co-trimoxazole and Erythromycin. Recommendations: One study conducted at Mwananyamala District Hospital in children aged 2 to 5 years is not enough to make a recommendation on the prevalence and pattern of organisms causing Urinary Tract Infection in children between two and five years in Dar es Salaam. It is proposed that further long term studies be conducted in order to produce results to advice government to change the current standard of antibiotic treatment for urinary tract infection.

Description

A dissertation submitted in partial fulfillment of the requirement for the degree of Master of Medicine (Pediatrics and Child Health) of the Hubert Kairuki Memorial University

Keywords

Antimicrobial, Urinary Tract Infection, Mwananyamala hospital

Citation

Barongo, A. K., 2013. Urinary tract infection: prevalence pathogens and antimicrobial susceptibility pattern among febrile children at Mwananyamala hospital Tanzania. HKMU

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