AmpFlSTR® IdentifilerTM STR allele frequencies in Tanzania, Africa.
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Date
2008-01
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of forensic sciences
Abstract
Allele frequencies of the fifteen AMPLFlSTRÒ IdentifilerTM
STR loci were determined for the population of Tanzania, East
Africa. Two hundred and seventy-two saliva samples were gath-
ered from unrelated healthy student or employee volunteers at
Muhimbili University College of Health Sciences in Dar es Sal-
aam, Tanzania during the summer of 2003. The tribal affiliation
of each donor was recorded to ensure that representative sam-
pling was achieved, and a total of 19 widely distributed adminis-
trative regions of the country and 42 tribes were represented in
the final genotypic data. DNA was extracted using the QIAamp
spin column protocol for saliva (Qiagen, Valencia, CA) and
quantitated using the QuantiblotÒ colorimetric system (Applied
Biosystems, Foster City, CA). Approximately 1 ng of DNA was
amplified from each sample using the IdentifilerTM
system
(Applied Biosystems) and subjected to capillary electrophoresis
on a 310 Genetic Analyzer (Applied Biosystems). Raw data was
collected, sorted, and sized using ABI Gene CollectionÒ and
GenescanÒ software (Applied Biosystems), and alleles were
assigned using ABI GenotyperÒ software (Applied Biosystems).
Allele frequencies were calculated using GENEPOPÓ online
software, v. 3.4 (1). Table 1 lists the frequencies of the alleles at
each locus, indicates the total number of samples used to generate
the table, and provides the database validation statistics.
A Fisher Exact Test was used to test adherence to Hardy Wein-
berg Equilibrium (HWE) at all loci (2,000 shufflings, GENEPOPÓ
online software, v 3.4). Twelve of the 15 loci yielded p-values of
>0.05, and thus failed to reject the hypothesis that the population is
in HWE at these loci. The remaining three loci, D8S1178,
D3S1358, and D2S1338, yielded p-values <0.05; however, a trun-
cated product test for adherence of all loci yielded a p-value of
0.0654, thus validating the database for general use (2).
Description
Keywords
Forensic science, DNA identification testing, Short tandem repeats
Citation
Forward, B.W., Eastman, M.W., Nyambo, T.B. and Ballard, R.E., 2008. AmpFlSTR® IdentifilerTM STR allele frequencies in Tanzania, Africa. J Forensic Sci, 53(1), pp.245-247.