AmpFlSTR® IdentifilerTM STR allele frequencies in Tanzania, Africa.

Nyambo, Thomas B.

AmpFlSTR® IdentifilerTM STR allele frequencies in Tanzania, Africa.

dc.contributor.author	Nyambo, Thomas B.
dc.date.accessioned	2023-09-26T06:00:09Z
dc.date.available	2023-09-26T06:00:09Z
dc.date.issued	2008-01
dc.description.abstract	Allele frequencies of the fifteen AMPLFlSTRÒ IdentifilerTM STR loci were determined for the population of Tanzania, East Africa. Two hundred and seventy-two saliva samples were gath- ered from unrelated healthy student or employee volunteers at Muhimbili University College of Health Sciences in Dar es Sal- aam, Tanzania during the summer of 2003. The tribal affiliation of each donor was recorded to ensure that representative sam- pling was achieved, and a total of 19 widely distributed adminis- trative regions of the country and 42 tribes were represented in the final genotypic data. DNA was extracted using the QIAamp spin column protocol for saliva (Qiagen, Valencia, CA) and quantitated using the QuantiblotÒ colorimetric system (Applied Biosystems, Foster City, CA). Approximately 1 ng of DNA was amplified from each sample using the IdentifilerTM system (Applied Biosystems) and subjected to capillary electrophoresis on a 310 Genetic Analyzer (Applied Biosystems). Raw data was collected, sorted, and sized using ABI Gene CollectionÒ and GenescanÒ software (Applied Biosystems), and alleles were assigned using ABI GenotyperÒ software (Applied Biosystems). Allele frequencies were calculated using GENEPOPÓ online software, v. 3.4 (1). Table 1 lists the frequencies of the alleles at each locus, indicates the total number of samples used to generate the table, and provides the database validation statistics. A Fisher Exact Test was used to test adherence to Hardy Wein- berg Equilibrium (HWE) at all loci (2,000 shufflings, GENEPOPÓ online software, v 3.4). Twelve of the 15 loci yielded p-values of >0.05, and thus failed to reject the hypothesis that the population is in HWE at these loci. The remaining three loci, D8S1178, D3S1358, and D2S1338, yielded p-values <0.05; however, a trun- cated product test for adherence of all loci yielded a p-value of 0.0654, thus validating the database for general use (2).	en_US
dc.identifier.citation	Forward, B.W., Eastman, M.W., Nyambo, T.B. and Ballard, R.E., 2008. AmpFlSTR® IdentifilerTM STR allele frequencies in Tanzania, Africa. J Forensic Sci, 53(1), pp.245-247.	en_US
dc.identifier.uri	doi: 10.1111/j.1556-4029.2007.00640.x
dc.identifier.uri	http://hdl.handle.net/123456789/1213
dc.language.iso	en	en_US
dc.publisher	Journal of forensic sciences	en_US
dc.subject	Forensic science	en_US
dc.subject	DNA identification testing	en_US
dc.subject	Short tandem repeats	en_US
dc.title	AmpFlSTR® IdentifilerTM STR allele frequencies in Tanzania, Africa.	en_US
dc.type	Article	en_US