Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa

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dc.contributor.authorMutabingwa, Theonest K.
dc.date.accessioned2020-03-11T04:29:05Z
dc.date.available2020-03-11T04:29:05Z
dc.date.issued2003
dc.description.abstractThe selection of point mutation at codon 164 (from isoleucine to leucine) of the dihydrofolate reductase (DHFR) enzyme in Plasmodium falciparum is associated with high sulfadoxine / pyrimethamine (SP) resistance. Using the yeast expression system that allows the detection of dhfr allele present at low level, the presence of this mutation had previously been reported between 1998–1999 in Muheza, Tanzania, an area of high SP resistance. Eighty five P. falciparum isolates, obtained from the same area between 2002 and 2003, were analysed for the presence of Leu-164 mutation, using standard protocol based on PCR-RFLP. None of the isolates had the Leu-164 mutation.en_US
dc.identifier.citationOchong, E., Nzila, A., Kimani, S., Kokwaro, G., Mutabingwa, T., Watkins, W. and Marsh, K., 2003. Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa. Malaria journal, 2(1), p.46.en_US
dc.identifier.otherDOIhttps://doi.org/10.1186/1475-2875-2-46
dc.identifier.urihttp://hdl.handle.net/123456789/319
dc.language.isoenen_US
dc.publisherMalaria journalen_US
dc.subjectMalariaen_US
dc.subjectArtesunateen_US
dc.subjectDihydrofolate reductaseen_US
dc.titleMolecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africaen_US
dc.typeArticleen_US

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