PCR targeting Plasmodium mitochondrial genome of DNA extracted from dried blood on filter paper compared to whole blood
| dc.contributor.author | Fataki, Maulidi R. | |
| dc.date.accessioned | 2020-02-26T10:37:35Z | |
| dc.date.available | 2020-02-26T10:37:35Z | |
| dc.date.issued | 2014 | |
| dc.description.abstract | Background Monitoring mortality and morbidity attributable to malaria is paramount to achieve elimination of 1,2 malaria. Diagnosis of malaria is challenging and PCR is a reliable method for identifying malaria with high sensitivity. However, blood specimen collection and transport can be challenging and obtaining dried blood spots (DBS) on filter paper by finger-prick may have advantages over collecting whole blood by venepuncture. Methods: DBS and whole blood were collected from febrile children admitted at the general paediatric wards at a referral hospital in Dar es Salaam, Tanzania. DNA extracted from whole blood and from DBS was tested with a genus-specific PCR targeting the mitochondrial Plasmodium genome. Positive samples by PCR of DNA from whole blood were tested with species-specific PCR targeting the 18S rRNA locus, or sequencing if species-specific PCR was negative. Rapid diagnostic test (RDT) and thin blood smear microscopy was carried out on all patients where remnant whole blood and a blood slide, respectively, were available. Results: Positivity of PCR was 24.5 (78/319) and 11.2% (52/442) by whole blood and DBS, respectively. All samples positive on DBS were also positive on Plasmodium falciparum species-specific PCR. All RDT positive cases were also positive by DBS PCR. All but three cases with positive blood slides were also positive by DBS. Conclusions: In this study, PCR for malaria mitochondrial DNA extracted from whole blood was more sensitive than from DBS. However, DBS are a practical alternative to whole blood and detected approximately the same number of cases as RDTs and, therefore, remain relevant for research purposes. | en_US |
| dc.identifier.citation | Strøm, G.E., Moyo, S., Fataki, M., Langeland, N. and Blomberg, B., 2014. PCR targeting Plasmodium mitochondrial genome of DNA extracted from dried blood on filter paper compared to whole blood. Malaria journal, 13(1), p.137. | en_US |
| dc.identifier.other | doi.org/10.1186/1475-2875-13-137 | |
| dc.identifier.uri | http://hdl.handle.net/123456789/277 | |
| dc.language.iso | en | en_US |
| dc.publisher | Malaria journal | en_US |
| dc.subject | Malaria | en_US |
| dc.subject | Filter paper | en_US |
| dc.subject | Dried blood spots/DBS | en_US |
| dc.title | PCR targeting Plasmodium mitochondrial genome of DNA extracted from dried blood on filter paper compared to whole blood | en_US |
| dc.type | Article | en_US |