Moshi, Mainen J.2023-08-152023-08-152019Omole, R.A., Moshi, M.J., Heydenreich, M., Malebo, H.M., Gathirwa, J.W., Oriko, R.O., Omosa, L.K. and Midiwo, J.O., 2019. Antiplasmodial Biflavanones from the Stem Bark of Garcinia buchananii Engl.DOI: 10.5530/pc.2019.3.20.http://hdl.handle.net/123456789/1155Introduction: Plants of the genus Garcinia are traditionally used treat a range of infectious and non-infectious diseases. Garcinia species are reported to have been shown to have a range of biological activities including cytotoxicity antimicrobial, antifungal, antioxidant, antimalarial and HIV-1 protease inhibitory activity among others. Methods: Solvent extraction was done using CH2Cl2: MeOH (1:1). Isolation was done using column chromatography with silica gel as the stationery phase and ethyl acetate and n-hexane used as mobile phase in increasing polarity. Thin layer chromatography was used to monitor the isolation. Structure elucida-tion was done using nuclear magnetic resonance and mass spectroscopic techniques. Chloroquine resistant (W2) and chloroquine sensitive (D6) P. falciparum strains were used for antiplasmodial assay. Results: Further bioassay guided fractionation of a CH2Cl2: MeOH (1:1) extract of Garcinia buchananii led to the isolation of two already reported biavanones, isogar-cinol (1) and guttiferone (2) with promising antiplasmodial activity against a chloroquine resistant (W2) Plasmodium falciparum strain with an IC50 of 2.8 ± 0.90 µg/mL for compound 1 and IC50 of 3.94 ± 0.38 µg/mL for compound 2. Compounds 1 and 2 also exhibited moderate activity against the chloro-quine sensitive (D6) Plasmodium falciparum strain with IC50 of 7.03±0.60 and 10.64±4.50 µg/mL, respectively. Conclusion: The results provide proof to support the use of G. buchananii by the indigenous community for anti-malarial therapy. Key words: Garcinia buchananii, Isogarcinol, Guttiferone F, Antiplasmodial activityenGarcinia buchananiiIsogarcinolAntiplasmodial activityArticle